줄기세포 및 응용 연구실 (LABORATORY OF STEM CELLS & APPLICATIONS)

연구주제 1

  • 인간 전분화능줄기세포 배양 및 분화 연구
  • 폐 오가노이드 및 대식세포 기반 폐섬유화증 및 감염 연구

PI 및 연구원 소개

  • 교  수 : 홍석호 Ph.D.
  • 연구원 : 정수지, 강민제, 정지혜, 조동욱, 김지영

연구실 소개

  • 인간 전분화능줄기세포 유래 폐상피세포, 혈액 및 혈관세포 분화 조절 연구
  • 폐섬유화증 치료 및 발병 기전 규명을 위한 신규 약물 유효성 평가 연구
  • 폐 오가노이드 및 대식세포 기반 질환모델, 감염 및 환경 유해물질 독성 평가 연구

연구 성과 소개

  • Diesel PM2.5 induces EMT and upregulation of SARS-CoV-2 receptor ACE2 during hPSC-derived alveolar organoid development. International Journal of Environmental Research & Public Health (2020)
  • Perivascular stem cell-derived CyPA improves uterine environment with AS via HIF1a-dependent angiogenesis. Molecular Therapy (2020)
  • BMP4 and perivascular cells regulate hematopoietic differentiation of hPSCs in a differentiation stage-specific manner. Experimental & Molecular Medicine (2020)
  • Human PSC-derived alveolar epithelial cells are alternative for in vitro pulmotoxicity assessment. Scientific Reports (2019)
  • Reprogramming mechanisms influence the maturation of hematopoietic cells from human pluripotent stem cells. Cell Death & Disease (2018)
  • Cadmium-induced inflammation & ER stress are mediated through C/EBP-DDIT3 signaling in human bronchial epithelial cells. Experimental & Molecular Medicine (2017)

연구주제 2

  • Bio-materials and stem cell engineering for pulmonary diseases

PI 및 연구원 소개

  • 교  수 : 양세란 D.V.M., Ph.D.
  • 박사과정 : 이주연
  • 석사과정 : 백효신, 장지민, 박재현
  • 학부생 : 차상률, 이세비, 최이설, 이윤도

연구실 소개

  • 바이오 소재 개발을 통한 난치성 질환 (급성폐손상, 만성폐쇄성폐질환 및 폐섬유화증) 치료 기술 개발
  • 줄기세포 기능강화 기술 개발
  • 줄기세포 유래 오가노이드를 통한 생활 속 화학물질 위해성 평가 플랫폼 개발

연구 성과 소개

  • Organoid Model in Idiopathic Pulmonary Fibrosis. Int J Stem Cells (2021)
  • WKYMVm ameliorates acute lung Injury via neutrophil antimicrobial peptide derived STAT1/IRF1 pathway. Biochem Biophys Res Commun (2020)
  • CMIT/MIT induce apoptosis and inflammation in alveolar epithelial cells through p38/JNK/ERK1/2 signaling pathway. Mol Cell Toxicol. (2020)
  • Inhibition of RAGE Attenuates Cigarette Smoke-Induced Lung Epithelial Cell Damage via RAGE-Mediated Nrf2/DAMP Signaling. Front Pharmacol. (2018)
  • Blockade of RAGE ameliorates elastase-induced emphysema development and progression via RAGE-DAMP signaling. FASEB J (2017)
  • Cigarette smoke-mediated oxidative stress induces apoptosis via the MAPKs/STAT1 pathway in mouse lung fibroblasts. Toxicol Lett (2016)
  • Reactive Oxygen Species in Mesenchymal Stem Cell Aging: Implication to Lung Diseases. Oxid Med Cell Longev (2015)

연구주제 3

  • The effect of mesenchymal stem cell on astrogliosis of CNS injury

PI 및 연구원 소개

  • 교  수 : 김충효 MD, Ph.D.
  • 공동연구 교수 : 이승진 MD, Ph.D., 김지하 MD, Ph.D.
  • 박사과정 : 방재승

연구실 소개

  • Aim: Safe clinical study of stem cells for SCI or TBI
    • Prove mechanism of recovery from SCI by stem cells
    • Modulation of acute gliosis could improve recovery of SCI
  • Stem cells
    • Neuronal priming bone marrow-derived mesenchymal stem cell of rat
    • Neuronal differentiation of human adipose tissue-derived MSCs
  • Target diseases
    • Spinal cord injury
    • Traumatic brain injury
    • Amyotrophic lateral sclerosis

연구 성과 소개

  • Mesenchymal Stem Cell Transplantation Promotes Functional Recovery through MMP2/STAT3 Related Astrogliosis after Spinal Cord Injury. Int J Stem Cells (2019)
  • Effects of Superparamagnetic Iron Oxide Nanoparticles on Essential Attributes Requested in Bone Marrow-Derived Mesenchymal Stem Cells Used for Neurological Disease Therapy. J Biomater Tissue Eng (2019)
  • An Increase in Mesenchymal Stem Cells Expressing Nestin in Bone-Marrow-Derived Primary Cells Stimulates Neurogenic Differentiation in Rat. The Korean Society of Embryo Transfer (2017)
  • Gelatin Directly Enhances Neurogenic Differentiation Potential in Bone Marrow-Derived Mesenchymal Stem Cells Without Stimulation of Neural Progenitor Cell Proliferation. DNA and Cell Biology (2016)
  • Mass Production of Early-Stage Bone-Marrow-Derived Mesenchymal Stem Cells of Rat Using Gelatin-Coated Matrix. BioMed Res Int. (2013)
  • Generation of priming mesenchymal stem cells with enhanced potential to differentiate into specific cell lineages using extracellular matrix proteins. Biochem and Biophys Res Commun (2013)